Validating internal controls for quantitative plant gene expression studies Horny bi chat rooms

Results A total of 35 Aux/IAA and 39 ARF genes were identified in the Populus genome...

Background Real-time reverse transcription PCR (RT-PCR) has greatly improved the ease and sensitivity of quantitative gene expression studies.

Additionally, using different rice cultivars, tissues and physiological conditions, we evaluated the expression stability of seven reference genes.However the usefulness of these is often limited by their sensitivity and accuracy, particularly for low-abundance transcripts.In contrast, quantitative reverse transcription – polymerase chain reaction (q RT-PCR or real-time RT-PCR) allows even weakly expressed genes to be accurately quantified [2].The functional characterization of TFs is crucial for the reconstruction of transcriptional regulatory networks controlling developmental and physiological processes such as growth, organ formation and the response to hormonal or environmental stimuli [7, 8].Transcription factor genes represent a sizable fraction of the genomes of all eukaryotic organisms, including higher plants [7].

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